Staff profile

Daphne Jackson Fellowship 2009 - 2011

• Biophysical Methods
• Crystallography
• Molecular Biology

Journal Article

• Jarrin, M., Kalligeraki, A. A., Uwineza, A., Cawood, C. S., Brown, A. P., Ward, E. N., Le, K., Freitag-Pohl, S., Pohl, E., Kiss, B., Tapodi, A., & Quinlan, R. A. (2023). Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix. Cells, 12(12), Article 1580. https://doi.org/10.3390/cells12121580
• Jasilionis, A., Plotka, M., Wang, L., Dorawa, S., Lange, J., Watzlawick, H., van den Bergh, T., Vroling, B., Altenbuchner, J., Kaczorowska, A., Pohl, E., Kaczorowski, T., Nordberg Karlsson, E., & Freitag‐Pohl, S. (2023). AmiP from hyperthermophilic Thermus parvatiensis prophage is a thermoactive and ultrathermostable peptidoglycan lytic amidase. Protein Science, 32(3), Article e4585. https://doi.org/10.1002/pro.4585
• Sowerby, K., Freitag-Pohl, S., Murillo, A. M., Silber, A. M., & Pohl, E. (2023). Cysteine synthase: multiple structures of a key enzyme in cysteine synthesis and a potential drug target for Chagas disease and leishmaniasis. Acta Crystallographica Section D: Structural Biology, 79(6), 518-530. https://doi.org/10.1107/s2059798323003613
• Andrews, J. L., Nilsson Lill, S. O., Freitag-Pohl, S., Apperley, D. C., Yufit, D. S., Batsanov, A. S., Mulvee, M. T., Edkins, K., McCabe, J. F., Berry, D. J., Probert, M. R., & Steed, J. W. (2021). Derisking the Polymorph Landscape: The Complex Polymorphism of Mexiletine Hydrochloride. Crystal Growth and Design, 21(12), 7150-7167. https://doi.org/10.1021/acs.cgd.1c01009
• Schwarz, M., Eno, R. F., Freitag-Pohl, S., Coxon, C. R., Straker, H. E., Wortley, D. J., Hughes, D. J., Mitchell, G., Moore, J., Cummins, I., Onkokesung, N., Brazier-Hicks, M., Edwards, R., Pohl, E., & Steel, P. G. (2021). Flavonoid-based inhibitors of the Phi-class glutathione transferase from black-grass to combat multiple herbicide resistance. Organic and Biomolecular Chemistry, 19(42), 9211-9222. https://doi.org/10.1039/d1ob01802g
• Aevarsson, A., Kaczorowska, A.-K., Adalsteinsson, B. T., Ahlqvist, J., Al-Karadaghi, S., Altenbuchner, J., Arsin, H., Atlasson, U. A., Brandt, D., Cichowicz-Cieslak, M., Cornish, K. A., Courtin, J., Dabrowski, S., Dahle, H., Djeffane, S., Dorawa, S., Dusaucy, J., Enault, F., Fedoy, A.-E., Freitag-Pohl, S., …Zhang, R. (2021). Going to extremes - a metagenomic journey into the dark matter of life. FEMS Microbiology Letters, 368(12), Article fnab067. https://doi.org/10.1093/femsle/fnab067
• Escrivani, D. O., Charlton, R. L., Caruso, M. B., Burle-Caldas, G. A., Borsodi, M. P. G., Zingali, R. B., Arruda-Costa, N., Palmeira-Mello, M. V., de Jesus, J. B., Souza, A. M., Abrahim-Vieira, B., Freitag-Pohl, S., Pohl, E., Denny, P. W., Rossi-Bergmann, B., & Steel, P. G. (2021). Chalcones identify cTXNPx as a potential antileishmanial drug target. PLoS Neglected Tropical Diseases, 15(11), Article e0009951. https://doi.org/10.1371/journal.pntd.0009951
• Zohib, M., Maheshwari, D., Pal, R. K., Freitag-Pohl, S., Biswal, B. K., Pohl, E., & Arora, A. (2020). Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani. Acta Crystallographica Section F: Structural Biology Communications, 76(11), 544-556. https://doi.org/10.1017/s2053230x20013722
• Freitag-Pohl, S., Jasilionis, A., Håkansson, M., Svensson, L. A., Kovačič, R., Welin, M., Watzlawick, H., Wang, L., Altenbuchner, J., Płotka, M., Kaczorowska, A. K., Kaczorowski, T., Nordberg Karlsson, E., Al-Karadaghi, S., Walse, B., Aevarsson, A., & Pohl, E. (2019). Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS. Acta Crystallographica Section D: Structural Biology, 75(11), 1028-1039. https://doi.org/10.1107/s2059798319013330
• Bruce, D., Cardew, E., Freitag-Pohl, S., & Pohl, E. (2019). How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project. Journal of Visualized Experiments, 144, Article e58666. https://doi.org/10.3791/58666
• Fleischer, R., Freitag, S., & Stalke, D. (2018). Oxidation of [Li-4\(NBut)(3)S\(2)]: a new route to sulfur triimides. Dalton Transactions, 193-198. https://doi.org/10.1039/a707085c
• Cummins, I., Dixon, D., Freitag-Pohl, S., Skipsey, M., & Edwards, R. (2011). Multiple roles for plant glutathione transferases in xenobiotic detoxification. Drug Metabolism Reviews, 43(2), 266-280. https://doi.org/10.3109/03602532.2011.552910
• Le Trong, I., Freitag, S., Klumb, L., Chu, V., Stayton, P., & Stenkamp, R. (2003). Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta crystallographica. Section D, Biological crystallography, 59(9), 1567-1573. https://doi.org/10.1107/s0907444903014562
• Hyre, D., Le Trong, I., Freitag, S., Stenkamp, R., & Stayton, P. (2000). Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system. Protein Science, 9(5), 878-885. https://doi.org/10.1110/ps.9.5.878
• Freitag, S., Chu, V., Penzotti, J., Klumb, L., To, R., Hyre, D., Le Trong, I., Lybrand, T., Stenkamp, R., & Stayton, P. (1999). A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway. Proceedings of the National Academy of Sciences, 96(15), 8384-8389. https://doi.org/10.1073/pnas.96.15.8384
• Freitag, S., Le Trong, I., Klumb, L., Chu, V., Chilkoti, A., Stayton, P., & Stenkamp, R. (1999). X-ray crystallographic studies of streptavidin mutants binding to biotin. https://doi.org/10.1016/s1050-3862%2899%2900048-0
• Stayton, P., Freitag, S., Klumb, L., Chilkoti, A., Chu, V., Penzotti, J., To, R., Hyre, D., Le Trong, I., Lybrand, T., & Stenkamp, R. (1999). Streptavidin-biotin binding energetics. https://doi.org/10.1016/s1050-3862%2899%2900042-x
• Freitag, S., Le Trong, I., Klumb, L., Stayton, P., & Stenkamp, R. (1999). Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?. Acta crystallographica. Section D, Biological crystallography, 55(6), 1118-1126. https://doi.org/10.1107/s0907444999002322
• Freitag, S., Le Trong, I., Chilkoti, A., Klumb, L., Stayton, P., & Stenkamp, R. (1998). Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. Journal of Molecular Biology, 279(1), 211-221. https://doi.org/10.1006/jmbi.1998.1735
• Chu, V., Freitag, S., Le Trong, I., Stenkamp, R., & Stayton, P. (1998). Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system. Protein Science, 7(4), 848-859
• Freitag, S., LeTrong, I., Klumb, L., Stayton, P., & Stenkamp, R. (1997). Structural studies of the streptavidin binding loop. Protein Science, 6(6), 1157-1166. https://doi.org/10.1002/pro.5560060604
• Ossig, G., Meller, A., Freitag, S., Muller, O., Gornitzka, H., & HerbstIrmer, R. (1996). Products of the reactions of 2-diazomalonate and of 2-diazoindanedione with germylenes and stannylenes. Organometallics, 15(1), 408-414. https://doi.org/10.1021/om950599p
• Freitag, S., HerbstIrmer, R., Lameyer, L., & Stalke, D. (1996). Observation of a Ge-Li bond: Donor-base-stabilized (tris(trimethylsilyl)germyl)lithium. Organometallics, 15(12), 2839-2841. https://doi.org/10.1021/om9600295
• Fleischer, R., Freitag, S., Pauer, F., & Stalke, D. (1996). [S(NtBu)(3)](2-) - A cap-shaped dianion, isoelectronic with the sulfite ion and oxidizable to a stable radical anion. Angewandte Chemie. International edition in English, 35(2), 204-206. https://doi.org/10.1002/anie.199602041
• Freitag, S., & HerbstIrmer, R. (1996). Crystal structure of chloro-dimethylstannyl-diphenylphosphinoyl-ethan-phosphonaciddiisopropyl ester, C22H33ClO4P2Sn. Zeitschrift für Kristallographie - Crystalline Materials, 211(11), 859-860. https://doi.org/10.1524/zkri.1996.211.11.859
• Hemme, I., Klingebiel, U., Freitag, S., & Stalke, D. (1995). Monomeric trilithium-tris[tert-butyldimethylsilylamido]phenylsilane, PhSi[NLi(thf)SiMe(2)CMe(3)](3) - Synthesis and crystal structure. https://doi.org/10.1002/zaac.19956211220
• FREITAG, S., Herbstirmer, R., Ahlemann, J., & Roesky, H. (1995). (N-(1-ADAMANTYL)[(PENTAFLUORO-2-PROPENYL)THIO]AMINO)(FLUORO)BIS[2,4, 6-TRIS(TRIFLUOROMETHYL)PHENYL]TIN AT 153 K. Acta crystallographica. Section C, Crystal structure communications, 51(4), 631-633. https://doi.org/10.1107/s0108270194011996
• Voelker, H., FREITAG, S., Pieper, U., & Roesky, H. (1995). SYNTHESIS OF THE NEW SILANEDIYLDIPHOSPHINITE TBU(2)SI(OPPH(2))(2) AND ITS REACTIONS WITH THE NORBORNADIENE COMPLEXES C(7)H(8)M(CO)(4) (M=CR, MO, W) - CRYSTAL-STRUCTURES OF CIS-M(CO)(4),[TBU(2)SI(OPPH(2))(2)] (M=CR, MO). https://doi.org/10.1002/zaac.19956210433
• FREITAG, S., Herbstirmer, R., Richter, F., & Weichmann, H. (1994). TETRAISOPROPYL 2-[(BROMO)(CHLORO)(METHYL)STANNYL]-1,1-ETHANEDIPHOSPHONATE AT 153K. Acta crystallographica. Section C, Crystal structure communications, 50(10), 1588-1590. https://doi.org/10.1107/s0108270194004105
• Drost, C., Jager, C., FREITAG, S., Klingebiel, U., Noltemeyer, M., & Sheldrick, G. (1994). DILITHIUM N,N'-BIS(TRIMETHYLSILYL)HYDRAZIDE AND A HYDROLYSIS PRODUCT - SIDE-ON-COORDINATED AND END-ON-COORDINATED LI CATIONS TO N2(2-) AND O2(2-). https://doi.org/10.1002/cber.19941270510
• FREITAG, S., Kolodziejski, W., Pauer, F., & Stalke, D. (1993). IMINOAMINOSULFINATES - SYNTHESIS, CRYSTAL-STRUCTURES, AND REARRANGEMENT MONITORED BY LI-7 SOLID-STATE NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY. Dalton Transactions, 3479-3488. https://doi.org/10.1039/dt9930003479
• Ossig, G., Meller, A., FREITAG, S., & Herbstirmer, R. (1993). SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF A STANNAIMINE. Journal of the Chemical Society. Chemical communications, 497-499. https://doi.org/10.1039/c39930000497
• Ossig, G., Meller, A., FREITAG, S., Herbstirmer, R., & Sheldrick, G. (1993). REACTIONS OF A FREE STANNAIMINE AND OF BASE-STABILIZED STANNYLENES. https://doi.org/10.1002/cber.19931261013
• Raubold, T., FREITAG, S., Herbstirmer, R., & Roesky, H. (1993). SYNTHESIS AND CRYSTAL-STRUCTURE OF THE SPIROCYCLE [(I-PR)2P(S)NSIME3]2SNCL2. https://doi.org/10.1002/zaac.19936190524
• Grutzmacher, H., FREITAG, S., Herbstirmer, R., & Sheldrick, G. (1992). INVESTIGATIONS OF THE STRUCTURE AND REACTIVITY OF A STANNAKETENIMINE. Angewandte Chemie. International edition in English, 31(4), 437-438. https://doi.org/10.1002/anie.199204371
• Meller, A., Ossig, G., Maringgele, W., Noltemeyer, M., Stalke, D., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1992). REACTIONS OF GERMYLENES WITH ACID AZIDES
• May, A., Roesky, H., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1992). [3 + 1] CYCLOADDITION - REACTION OF DICHLOROGERMYLENE WITH HEXAFLUORO-2-PROPANETHIONE 1-ADAMANTYLIMIDE. Organometallics, 11(1), 15-16. https://doi.org/10.1021/om00037a008
• Meller, A., Ossig, G., Maringgele, W., Stalke, D., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1991). SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF GERMAIMINES. Journal of the Chemical Society. Chemical communications, 1123-1124. https://doi.org/10.1039/c39910001123