Staff profile

Journal Article

• Drug and siRNA screens identify ROCK2 as a therapeutic target for ciliopathies
  
  Smith, C. E., Streets, A. J., Lake, A. V., Natarajan, S., Best, S. K., Szymanska, K., Karwatka, M., Stevenson, T., Trowbridge, R., Grant, G., Grellscheid, S. N., Foster, R., Morrison, C. G., Mavria, G., Bond, J., Ong, A. C., & Johnson, C. A. (2025). Drug and siRNA screens identify ROCK2 as a therapeutic target for ciliopathies. Communications Medicine, 5, Article 129. https://doi.org/10.1038/s43856-025-00847-1
• Deciphering the impact of PROM1 alternative splicing on human photoreceptor development and maturation.
  
  Moya-Molina, M., Dorgau, B., Flood, E., Letteboer, S. J. F., Lorentzen, E., Coxhead, J., Smith, G., Roepman, R., Nagaraja Grellscheid, S., Armstrong, L., & Lako, M. (2024). Deciphering the impact of PROM1 alternative splicing on human photoreceptor development and maturation. Cell Death & Disease, 15(10), Article 721. https://doi.org/10.1038/s41419-024-07105-7
• PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5´-splice-site selection causing tissue-specific defects.
  
  Atkinson, R., Georgiou, M., Yang, C., Szymanska, K., Lahat, A., Vasconcelos, E. J. R., Ji, Y., Moya Molina, M., Collin, J., Queen, R., Dorgau, B., Watson, A., Kurzawa-Akanbi, M., Laws, R., Saxena, A., Shyan Beh, C., Siachisumo, C., Goertler, F., Karwatka, M., … Lako, M. (2024). PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5´-splice-site selection causing tissue-specific defects. Nature Communications, 15(1), Article 3138. https://doi.org/10.1038/s41467-024-47253-0
• A bending rigidity parameter for stress granule condensates
  
  Law, J. O., Jones, C. M., Stevenson, T., Williamson, T. A., Turner, M. S., Kusumaatmaja, H., & Grellscheid, S. N. (2023). A bending rigidity parameter for stress granule condensates. Science Advances, 9(20). https://doi.org/10.1126/sciadv.adg0432
• MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control
  
  Thompson, H., Shen, W., Matus, R., Kakkar, M., Jones, C., Dolan, D., Grellscheid, S., Yang, X., Zhang, N., Mozaffari-Jovin, S., Chen, C., Zhang, X., Topping, J., & Lindsey, K. (2023). MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control. Development, 150(7), Article dev201476. https://doi.org/10.1242/dev.201476
• Peptide‐Based Coacervate‐Core Vesicles with Semipermeable Membranes
  
  Abbas, M., Law, J. O., Grellscheid, S. N., Huck, W. T., & Spruijt, E. (2022). Peptide‐Based Coacervate‐Core Vesicles with Semipermeable Membranes. Advanced Materials, 34(34), Article 2202913. https://doi.org/10.1002/adma.202202913
• Particle detection and tracking with DNA
  
  O’Hare, C. A., Matsos, V. G., Newton, J., Smith, K., Hochstetter, J., Jaiswar, R., Kyaw, W., McNamara, A., Kuncic, Z., Grellscheid, S. N., & Bœhm, C. (2022). Particle detection and tracking with DNA. The European Physical Journal C, 82(4), Article 306. https://doi.org/10.1140/epjc/s10052-022-10264-6
• Neutrophils induce paracrine telomere dysfunction and senescence in ROS‐dependent manner
  
  Lagnado, A., Leslie, J., Ruchaud‐Sparagano, M., Victorelli, S., Hirsova, P., Ogrodnik, M., Collins, A. L., Vizioli, M. G., Habiballa, L., Saretzki, G., Evans, S. A., Salmonowicz, H., Hruby, A., Geh, D., Pavelko, K. D., Dolan, D., Reeves, H. L., Grellscheid, S., Wilson, C. H., … Passos, J. F. (2021). Neutrophils induce paracrine telomere dysfunction and senescence in ROS‐dependent manner. The EMBO Journal, 40(9), Article e106048. https://doi.org/10.15252/embj.2020106048
• Deep conservation of ribosome stall sites across RNA processing genes
  
  Chyżyńska, K., Labun, K., Jones, C., Grellscheid, S. N., & Valen, E. (2021). Deep conservation of ribosome stall sites across RNA processing genes. NAR Genomics and Bioinformatics, 3(2). https://doi.org/10.1093/nargab/lqab038
• Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation
  
  Vietri, M., Schultz, S. W., Bellanger, A., Jones, C. M., Petersen, L. I., Raiborg, C., Skarpen, E., Pedurupillay, C. R. J., Kjos, I., Kip, E., Timmer, R., Jain, A., Collas, P., Knorr, R. L., Grellscheid, S. N., Kusumaatmaja, H., Brech, A., Micci, F., Stenmark, H., & Campsteijn, C. (2020). Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation. Nature Cell Biology, 22(7), 856-867. https://doi.org/10.1038/s41556-020-0537-5
• The bystander effect contributes to the accumulation of senescent cells in vivo
  
  da Silva, P. F., Ogrodnik, M., Kucheryavenko, O., Glibert, J., Miwa, S., Cameron, K., Ishaq, A., Saretzki, G., Nagaraja-Grellscheid, S., Nelson, G., & von Zglinicki, T. (2019). The bystander effect contributes to the accumulation of senescent cells in vivo. Aging Cell, 18(1), Article e12848. https://doi.org/10.1111/acel.12848
• An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning
  
  Ehrmann, I., Crichton, J. H., Gazzara, M. R., James, K., Liu, Y., Grellscheid, S. N., Curk, T., de Rooij, D., Steyn, J. S., Cockell, S., Adams, I. R., Barash, Y., & Elliott, D. J. (2019). An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. ELife, 8, Article e39304. https://doi.org/10.7554/elife.39304
• An integrated transcriptional analysis of the developing human retina
  
  Mellough, C. B., Bauer, R., Collin, J., Dorgau, B., Zerti, D., Dolan, D. W., Jones, C. M., Izuogu, O. G., Yu, M., Hallam, D., Steyn, J. S., White, K., Steel, D. H., Santibanez-Koref, M., Elliott, D. J., Jackson, M. S., Lindsay, S., Grellscheid, S., & Lako, M. (2019). An integrated transcriptional analysis of the developing human retina. Development., 146(2), Article dev169474. https://doi.org/10.1242/dev.169474
• Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa
  
  Buskin, A., Zhu, L., Chichagova, V., Basu, B., Mozaffari-Jovin, S., Dolan, D., Droop, A., Collin, J., Bronstein, R., Mehrotra, S., Farkas, M., Hilgen, G., White, K., Pan, K.-T., Treumann, A., Hallam, D., Bialas, K., Chung, G., Mellough, C., … Lako, M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9(1), Article 4234. https://doi.org/10.1038/s41467-018-06448-y
• Medicinal Mascarene Aloe s: An audit of their phytotherapeutic potential
  
  Lobine, D., Cummins, I., Govinden-Soulange, J., Ranghoo-Sanmukhiya, M., Lindsey, K., Chazot, P., Ambler, C., Grellscheid, S., Sharples, G., Lall, N., Lambrechts, I., Lavergne, C., & Howes, M.-J. (2018). Medicinal Mascarene Aloe s: An audit of their phytotherapeutic potential. Fitoterapia, 124, 120-126. https://doi.org/10.1016/j.fitote.2017.10.010
• Cellular senescence drives age-dependent hepatic steatosis
  
  Ogrodnik, M., Miwa, S., Tchkonia, T., Tiniakos, D., Wilson, C. L., Lahat, A., Day, C. P., Burt, A., Palmer, A., Anstee, Q. M., Grellscheid, S. N., Hoeijmakers, J. H., Barnhoorn, S., Mann, D. A., Bird, T. G., Vermeij, W. P., Kirkland, J. L., Passos, J. F., von Zglinicki, T., & Jurk, D. (2017). Cellular senescence drives age-dependent hepatic steatosis. Nature Communications, 8. https://doi.org/10.1038/ncomms15691
• A tale of two paralogs: human Transformer2 proteins with differential RNA-binding affinities
  
  Ghosh, P., Grellscheid, S. N., & Sowdhamini, R. (2016). A tale of two paralogs: human Transformer2 proteins with differential RNA-binding affinities. Journal of Biomolecular Structure and Dynamics, 34(9), 1979-1986. https://doi.org/10.1080/07391102.2015.1100551
• Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68
  
  Feracci, M., Foot, J. N., Grellscheid, S. N., Danilenko, M., Stehle, R., Gonchar, O., Kang, H.-S., Dalgliesh, C., Meyer, N. H., Liu, Y., Lahat, A., Sattler, M., Eperon, I. C., Elliott, D. J., & Dominguez, C. (2016). Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nature Communications, 7, Article 10355. https://doi.org/10.1038/ncomms10355
• Comparison of senescence-associated miRNAs in primary skin and lung fibroblasts
  
  Holly, A., Grellscheid, S., van de Walle, P., Dolan, D., Pilling, L., Daniels, D., von Zglinicki, T., Ferrucci, L., Melzer, D., & Harries, L. (2015). Comparison of senescence-associated miRNAs in primary skin and lung fibroblasts. Biogerontology, 16, 423–434. https://doi.org/10.1007/s10522-015-9560-5
• TSC1 activates TGF-β-Smad2/3 signaling in growth arrest and epithelial-to-mesenchymal transition.
  
  Thien, A., Prentzell, M., Holzwarth, B., Kläsener, K., Kuper, I., Boehlke, C., Sonntag, A., Ruf, S., Maerz, L., Nitschke, R., Grellscheid, S., Reth, M., Walz, G., Baumeister, R., Neumann-Haefelin, E., & Thedieck, K. (2015). TSC1 activates TGF-β-Smad2/3 signaling in growth arrest and epithelial-to-mesenchymal transition. Developmental Cell, 32(5), 617-630. https://doi.org/10.1016/j.devcel.2015.01.026
• Detecting translational regulation by change point analysis of ribosome profiling data sets
  
  Zupanic, A., Meplan, C., Grellscheid, S., Mathers, J., Kirkwood, T., Hesketh, J., & Shanley, D. (2014). Detecting translational regulation by change point analysis of ribosome profiling data sets. RNA, 20(10), 1507-1518. https://doi.org/10.1261/rna.045286.114
• The splicing landscape is globally reprogrammed during male meiosis
  
  Schmid, R., Grellscheid, S., Ehrmann, I., Dalgliesh, C., Danilenko, M., Paronetto, M., Pedrotti, S., Grellscheid, D., Dixon, R., Sette, C., Eperon, I., & Elliott, D. (2013). The splicing landscape is globally reprogrammed during male meiosis. Nucleic Acids Research, 41(22), 10170-10184. https://doi.org/10.1093/nar/gkt811
• Inhibition of mTORC1 by Astrin and Stress Granules Prevents Apoptosis in Cancer Cells.
  
  Thedieck, K., Holzwarth, B., Prentzell, M., Boehlke, C., Kläsener, K., Ruf, S., Sonntag, A., Maerz, L., Grellscheid, S., Kremmer, E., Nitschke, R., Kuehn, E., Jonker, J., Groen, A., Reth, M., Hall, M., & Baumeister, R. (2013). Inhibition of mTORC1 by Astrin and Stress Granules Prevents Apoptosis in Cancer Cells. Cell, 154(4), 859-874. https://doi.org/10.1016/j.cell.2013.07.031
• How does Tra2β protein regulate tissue-specific RNA splicing?
  
  Elliott, D., Best, A., Dalgliesh, C., Ehrmann, I., & Grellscheid, S. (2012). How does Tra2β protein regulate tissue-specific RNA splicing?. Biochemical Society Transactions, 40(4), 784-788. https://doi.org/10.1042/bst20120036
• Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development
  
  Grellscheid, S., Dalgliesh, C., Storbeck, M., Best, A., Liu, Y., Jakubik, M., Mende, Y., Ehrmann, I., Curk, T., Rossbach, K., Bourgeois, C. F., Stévenin, J., Grellscheid, D., Jackson, M. S., Wirth, B., & Elliott, D. J. (2011). Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development. PLoS Genetics, 7(12), Article e1002390. https://doi.org/10.1371/journal.pgen.1002390
• Identification of novel androgen-regulated pathways and mRNA isoforms through genome-wide exon-specific profiling of the LNCaP transcriptome
  
  Rajan, P., Dalgliesh, C., Carling, P., Buist, T., Zhang, C., Grellscheid, S., Armstrong, K., Stockley, J., Simillion, C., Gaughan, L., Kalna, G., Zhang, M., Robson, C., Leung, H., & Elliott, D. (2011). Identification of novel androgen-regulated pathways and mRNA isoforms through genome-wide exon-specific profiling of the LNCaP transcriptome. PLoS ONE, 6(12), Article e29088. https://doi.org/10.1371/journal.pone.0029088
• Molecular design of a splicing switch responsive to the RNA binding protein Tra2β
  
  Grellscheid, S., Dalgliesh, C., Rozanska, A., Grellscheid, D., Bourgeois, C., Stévenin, J., & Elliott, D. (2011). Molecular design of a splicing switch responsive to the RNA binding protein Tra2β. Nucleic Acids Research, 39(18), 8092-8104. https://doi.org/10.1093/nar/gkr495
• Nonsense-mediated messenger RNA decay of survival motor neuron 1 causes spinal muscular atrophy.
  
  Brichta, L., Garbes, L., Jedrzejowska, M., Grellscheid, S., Holker, I., Zimmermann, K., & Wirth, B. (2008). Nonsense-mediated messenger RNA decay of survival motor neuron 1 causes spinal muscular atrophy. Human Genetics, 123(2), 141-153. https://doi.org/10.1007/s00439-007-0455-7
• Applying genetic programming to the prediction of alternative mRNA splice variants.
  
  Vukusic, I., Grellscheid, S., & Wiehe, T. (2007). Applying genetic programming to the prediction of alternative mRNA splice variants. Genomics, 89((4):), 471-479.
• The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction.
  
  Skrisovska, L., Bourgeois, C., Stefl, R., Grellscheid, S., Kister, L., Wenter, P., Elliott, D., Stevenin, J., & Allain, F. (2007). The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction. EMBO Reports, 8(4), 372-379. https://doi.org/10.1038/sj.embor.7400910
• An apparent pseudo-exon acts both as an alternative exon that leads to nonsense-mediated decay and as a zero-length exon.
  
  Grellscheid, S., & Smith, C. (2006). An apparent pseudo-exon acts both as an alternative exon that leads to nonsense-mediated decay and as a zero-length exon. Mol Cell Biol, 26((6):), 2237-2246.