Staff profile

Research Interests

My research centres on the biomolecular design approach to synthetic biology. Current directions include:

• The design of a novel protein based motor that walks along DNA.
• The design of sequences that adopt multiple native states.
• Peptide based methods for coupling changes in chemical potential to mechanical outputs.
• Understanding the energy landscape of coiled-coil oligomerization. 
• The design of self-assembling fibre systems.

 For further information please see here.

Journal Article

• Toxin release by conditional remodelling of ParDE1 from Mycobacterium tuberculosis leads to gyrase inhibition
  
  Beck, I. N., Arrowsmith, T. J., Grobbelaar, M. J., Bromley, E. C., Marles-Wright, J., & Blower, T. R. (2024). Toxin release by conditional remodelling of ParDE1 from Mycobacterium tuberculosis leads to gyrase inhibition. Nucleic Acids Research, 52(4), 1909-1929. https://doi.org/10.1093/nar/gkad1220
• Recurrent depression and relational trauma: a single case of memory processing
  
  Barton, S. B., Armstrong, P. V., Meares, K., Bromley, E. H., & Whitton, D. (2024). Recurrent depression and relational trauma: a single case of memory processing. The Cognitive Behaviour Therapist, 17, Article e14. https://doi.org/10.1017/s1754470x24000126
• CBT for difficult-to-treat depression: self-regulation model
  
  Barton, S. B., Armstrong, P. V., Robinson, L. J., & Bromley, E. H. (2023). CBT for difficult-to-treat depression: self-regulation model. Behavioural and Cognitive Psychotherapy, 51(6), 543-558. https://doi.org/10.1017/s1352465822000273
• Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome
  
  Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., Brown, A., Hodgson, D., Blower, T., Verlet, J., Degiacomi, M., Palsson, L.-O., & Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6
• Threaded Rings that Swim in Excitable Media
  
  Cincotti, A., Maucher, F., Evans, D., Chapin, B. M., Horner, K., Bromley, E., Lobb, A., Steed, J. W., & Sutcliffe, P. (2019). Threaded Rings that Swim in Excitable Media. Physical Review Letters, 123(25), Article 258102. https://doi.org/10.1103/physrevlett.123.258102
• The Bar-Hinge Motor: A synthetic protein design exploiting conformational switching to achieve directional motility
  
  Small, L., Zuckermann, M., Sessions, R., Curmi, P., Linke, H., Forde, N., & Bromley, E. (2019). The Bar-Hinge Motor: A synthetic protein design exploiting conformational switching to achieve directional motility. New Journal of Physics, 21, Article 013002. https://doi.org/10.1088/1367-2630/aaf3ca
• Stabilising Peptoid Helices Using Non‐Chiral Fluoroalkyl Monomers
  
  Gimenez, D., Aguilar, J., Bromley, E., & Cobb, S. (2018). Stabilising Peptoid Helices Using Non‐Chiral Fluoroalkyl Monomers. Angewandte Chemie International Edition, 57(33), 10549-10553. https://doi.org/10.1002/anie.201804488
• Excited-State Aromatic Interactions in the Aggregation-Induced Emission of Molecular Rotors
  
  Sturala, J., Etherington, M. K., Bismillah, A. N., Higginbotham, H. F., Trewby, W. J., Aguilar, J. A., Bromley, E. H., Avestro, A.-J., Monkman, A. P., & McGonigal, P. R. (2017). Excited-State Aromatic Interactions in the Aggregation-Induced Emission of Molecular Rotors. Journal of the American Chemical Society, 139(49), 17882-17889. https://doi.org/10.1021/jacs.7b08570
• Construction of a chassis for a tripartite protein-based molecular motor
  
  Small, L. S., Bruning, M., Thomson, A. R., Boyle, A. L., Davies, R. B., Curmi, P. M., Forde, N. R., Linke, H., Woolfson, D. N., & Bromley, E. H. (2017). Construction of a chassis for a tripartite protein-based molecular motor. ACS Synthetic Biology, 6(6), 1096-1102. https://doi.org/10.1021/acssynbio.7b00037
• Log D versus HPLC derived hydrophobicity: The development of predictive tools to aid in the rational design of bioactive peptoids
  
  Bolt, H., Williams, C., Brooks, R., Zuckermann, R., Cobb, S., & Bromley, E. (2017). Log D versus HPLC derived hydrophobicity: The development of predictive tools to aid in the rational design of bioactive peptoids. Biopolymers, 108(4), Article e23014. https://doi.org/10.1002/bip.23014
• Structural characterization suggests models for monomeric and dimeric forms of full-length ezrin
  
  Phang, J., Harrop, S., Duff, A., Sokolova, A., Crossett, B., Walsh, J., Beckham, S., Nguyen, C., Davies, R., Glöckner, C., Bromley, E., Wilk, K., & Curmi, P. (2016). Structural characterization suggests models for monomeric and dimeric forms of full-length ezrin. Biochemical Journal, 473(18), 2763-2782. https://doi.org/10.1042/bcj20160541
• Motor properties from persistence: a linear molecular walker lacking spatial and temporal asymmetry
  
  Zuckermann, M., Angstmann, C., Schmitt, R., Blab, G., Bromley, E., Forde, N., Linke, H., & Curmi, P. (2015). Motor properties from persistence: a linear molecular walker lacking spatial and temporal asymmetry. New Journal of Physics, 17(5), Article 055017. https://doi.org/10.1088/1367-2630/17/5/055017
• Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA
  
  Kovacic, S., Samii, L., Lamour, G., Li, H., Linke, H., Bromley, E., Woolfson, D., Curmi, P., & Forde, N. (2014). Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. Biomacromolecules, 15(11). https://doi.org/10.1021/bm501109p
• A Basis Set of de Novo Coiled-Coil Peptide Oligomers for Rational Protein Design and Synthetic Biology.
  
  Fletcher, J., Boyle, A., Bruning, M., Bartlett, G., Vincent, T., Zaccai, N., Armstrong, C., Bromley, E., Booth, P., Brady, R., Thomson, A., & Woolfson, D. (2012). A Basis Set of de Novo Coiled-Coil Peptide Oligomers for Rational Protein Design and Synthetic Biology. American Chemical Society Synthetic Biology, 1(6), 240-250. https://doi.org/10.1021/sb300028q
• Squaring the Circle in Peptide Assembly: From Fibers to Discrete Nanostructures by de Novo Design
  
  Boyle, A., Bromley, E., Bartlett, G., Sessions, R., Sharp, T., Williams, C., Curmi, P., Forde, N., Linke, H., & Woolfson, D. (2012). Squaring the Circle in Peptide Assembly: From Fibers to Discrete Nanostructures by de Novo Design. Journal of the American Chemical Society, 134(37). https://doi.org/10.1021/ja3053943
• Tuning the performance of an artificial protein motor.
  
  Kuwada, N., Zuckermann, M., Bromley, E., Sessions, R., Curmi, P., Forde, N., Woolfson, D., & Linke, H. (2011). Tuning the performance of an artificial protein motor. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 84(3), Article 031922. https://doi.org/10.1103/physreve.84.031922
• Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a Gram-negative bacterium.
  
  Xiong, X., Bromley, E., Oelschlaeger, P., Woolfson, D., & Spencer, J. (2011). Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a Gram-negative bacterium. Nucleic Acids Research, 39(9), 3917-3927. https://doi.org/10.1093/nar/gkq1296
• Crystal Structure of Serratia fonticola Sfh-I: Activation of the Nucleophile in Mono-Zinc Metallo-beta-Lactamases
  
  Fonseca, F., Bromley, E., Saavedra, M., Correia, A., & Spencer, J. (2011). Crystal Structure of Serratia fonticola Sfh-I: Activation of the Nucleophile in Mono-Zinc Metallo-beta-Lactamases. Journal of Molecular Biology, 411(5), 951-959. https://doi.org/10.1016/j.jmb.2011.06.043
• Time-dependent motor properties of multipedal molecular spiders
  
  Samii, L., Blab, G., Bromley, E., Linke, H., Curmi, P., Zuckermann, M., & Forde, N. (2011). Time-dependent motor properties of multipedal molecular spiders. Physical Review E, 84(3), Article 031111. https://doi.org/10.1103/physreve.84.031111
• Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-beta-Lactamase from Serratia fonticola UTAD54
  
  Fonseca, F., Arthur, C., Bromley, E., Samyn, B., Moerman, P., Saavedra, M., Correia, A., & Spencer, J. (2011). Biochemical Characterization of Sfh-I, a Subclass B2 Metallo-beta-Lactamase from Serratia fonticola UTAD54. Antimicrobial Agents and Chemotherapy, 55(11), 5392-5395. https://doi.org/10.1128/aac.00429-11
• Assembly Pathway of a Designed α-Helical Protein Fiber.
  
  Bromley, E., Channon, K., King, P., Mahmoud, Z., Banwell, E., Butler, M., Crump, M., Dafforn, T., Hicks, M., Hirst, J., Rodger, A., & Woolfson, D. (2010). Assembly Pathway of a Designed α-Helical Protein Fiber. Biophysical Journal, 98(8), 1668-1676. https://doi.org/10.1016/j.bpj.2009.12.4309
• Designed alpha-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems
  
  Bromley, E., Sessions, R., Thomson, A., & Woolfson, D. (2009). Designed alpha-Helical Tectons for Constructing Multicomponent Synthetic Biological Systems. Journal of the American Chemical Society, 131(3), 928-930. https://doi.org/10.1021/ja804231a
• Flow Linear Dichroism of Some Prototypical Proteins
  
  Bulheller, B., Rodger, A., Hicks, M., Dafforn, T., Serpell, L., Marshall, K., Bromley, E., King, P., Channon, K., Woolfson, D., & Hirst, J. (2009). Flow Linear Dichroism of Some Prototypical Proteins. Journal of the American Chemical Society, 131(37), 13305-13314.
• The Tumbleweed: towards a synthetic protein motor
  
  Bromley, E., Kuwada, N., Zuckermann, M., Donadini, R., Samii, L., Blab, G., Gemmen, G., Lopez, B., Curmi, P., Forde, N., Woolfson, D., & Linke, H. (2009). The Tumbleweed: towards a synthetic protein motor. HFSP Journal, 3(3), 204-212.
• Rational design of peptide-based building blocks for nanoscience and synthetic biology
  
  Armstrong, C., Boyle, A., Bromley, E., Mahmoud, Z., Smith, L., Thomson, A., & Woolfson, D. (2009). Rational design of peptide-based building blocks for nanoscience and synthetic biology. Faraday Discussions, 143, 305-317.
• Electrostatic control of thickness and stiffness in a designed protein fiber
  
  Papapostolou, D., Bromley, E., Bano, C., & Woolfson, D. (2008). Electrostatic control of thickness and stiffness in a designed protein fiber. Journal of the American Chemical Society, 130(15), 5124-5130. https://doi.org/10.1021/ja0778444
• Synthetic biology through biomolecular design and engineering
  
  Channon, K., Bromley, E., & Woolfson, D. (2008). Synthetic biology through biomolecular design and engineering. Current Opinion in Structural Biology, 18(4), 491-498.
• MagicWand: A single, designed peptide that assembles to stable, ordered alpha-helical fibers
  
  Gribbon, C., Channon, K., Zhang, W., Banwell, E., Bromley, E., Chaudhuri, J., Oreffo, R., & Woolfson, D. (2008). MagicWand: A single, designed peptide that assembles to stable, ordered alpha-helical fibers. Biochemistry., 47(39), 10365-10371.
• Peptide and protein building blocks for synthetic biology: From programming biomolecules to self-organized biomolecular systems
  
  Bromley, E., Channon, K., Moutevelis, E., & Woolfson, D. (2008). Peptide and protein building blocks for synthetic biology: From programming biomolecules to self-organized biomolecular systems. ACS Chemical Biology, 3(1), 38-50. https://doi.org/10.1021/cb700249v
• Optical microscopy of growing insulin amyloid spherulites on surfaces in vitro
  
  Rogers, S., Krebs, M., Bromley, E., van der Linden, E., & Donald, A. (2006). Optical microscopy of growing insulin amyloid spherulites on surfaces in vitro. Biophysical Journal, 90(3), 1043-1054.
• Mechanisms of structure formation in particulate gels of beta-lactoglobulin formed near the isoelectric point
  
  Bromley, E., Krebs, M., & Donald, A. (2006). Mechanisms of structure formation in particulate gels of beta-lactoglobulin formed near the isoelectric point. European Physical Journal E: Soft Matter and Biological Physics, 21(2), 145-152.
• Aggregation across the length-scales in beta-lactoglobulin
  
  Bromley, E., Krebs, M., & Donald, A. (2005). Aggregation across the length-scales in beta-lactoglobulin. Faraday Discussions, 128, 13-27.
• The mechanism of amyloid spherulite formation by bovine insulin
  
  Krebs, M., Bromley, E., Rogers, S., & Donald, A. (2005). The mechanism of amyloid spherulite formation by bovine insulin. Biophysical Journal, 88(3), 2013-2021.
• The binding of thioflavin-T to amyloid fibrils: localisation and implications
  
  Krebs, M., Bromley, E., & Donald, A. (2005). The binding of thioflavin-T to amyloid fibrils: localisation and implications. Journal of Structural Biology, 149(1), 30-37. https://doi.org/10.1016/j.jsb.2004.08.002
• L55P transthyretin accelerates subunit exchange and leads to rapid formation of hybrid tetramers
  
  Keetch, C., Bromley, E., McCammon, M., Wang, N., Christodoulou, J., & Robinson, C. (2005). L55P transthyretin accelerates subunit exchange and leads to rapid formation of hybrid tetramers. Journal of Biological Chemistry, 280(50), 41667-41674.
• Confocal microscopy of a dense particle system
  
  Bromley, E., & Hopkinson, I. (2002). Confocal microscopy of a dense particle system. Journal of Colloid and Interface Science., 245(1), 75-80.